Sensitive High-Throughput Assays for Tumour Burden Reveal the Response of a Drosophila melanogaster Model of Colorectal Cancer to Standard Chemotherapies

Drosophila melanogaster (Drosophila) models of cancer are emerging as powerful tools to investigate the basic mechanisms underlying tumour progression and identify novel therapeutics. Rapid and inexpensive, it is possible to carry out genetic and drug screens at a far larger scale than in vertebrate organisms. Such whole-organism-based drug screens permits assessment of drug absorption and toxicity, reducing the possibility of false positives. Activating mutations in the Wnt and Ras signalling pathways are common in many epithelial cancers, and when driven in the adult Drosophila midgut, it induces aggressive intestinal tumour-like outgrowths that recapitulate many aspects of human colorectal cancer (CRC). Here we have taken a Drosophila CRC model in which tumourous cells are marked with both GFP and luciferase reporter genes, and developed novel high-throughput assays for quantifying tumour burden. Leveraging these assays, we find that the Drosophila CRC model responds rapidly to treatment with standard CRC-drugs, opening the door to future rapid genetic and drug screens.     

One-Sided Chronic Intervillositis of Unknown Etiology in Dizygotic Twins: A Description of 3 Cases

Chronic intervillositis of unknown etiology (CIUE) is a rare, poorly understood, histopathological diagnosis of the placenta that is frequently accompanied by adverse pregnancy outcomes including miscarriage, fetal growth restriction, and intrauterine fetal death. CIUE is thought to have an immunologically driven pathophysiology and may be related to human leukocyte antigen mismatches between the mother and the fetus. Dizygotic twins with one-sided CIUE provide an interesting context to study the influence of immunogenetic differences in such cases. The main immune-cell subsets were investigated using immunohistochemistry. We identified three dizygotic twin pregnancies in which CIUE was present in only one of the two placentas. Two of the pregnancies ended in term delivery and one ended in preterm delivery. Presence of CIUE was correlated with lower placental weight and lower birthweight. Relative number of CD68, CD56, CD20, and CD3 positive cells were comparable between co-twins. The presence of one-sided CIUE in dizygotic twin pregnancy was associated with selective growth restriction in the affected twin. This suggests a unique fetal immunogenetic contribution to the pathogenesis of CIUE. Further study of dizygotic and monozygotic placentas affected by CIUE could identify new insights into its pathophysiology and into the field of reproductive immunology.     

Palestinian agriculture and the Israeli separation barrier: the mismatch of biopolitics and chronopolitics with the environment and human survival

Academic scholarship on Israel’s Separation Barrier has focused upon its legality and political impacts. United Nations agencies and Non-Governmental Organisations have been left to document its other actual/potential infringements. The natural environment has been secondary to higher profile issues, such as the intentions behind the structure and its implications for any future peace agreement. Yet, since environmental impediments can have serious long-term implications for natural resources and human subsistence, it is necessary to examine the barrier’s impacts after a decade of existence. This paper focuses upon the main human-environment system of agriculture. A multi-method approach reveals that the impacts constitute much more than the ‘population here, natural resources there’ thesis that has dominated narratives about the barrier’s environmental impediments. In fact, the barrier appears to be having dramatic and perhaps unexpected socio-ecological consequences.     

Comparison of functional outcomes and treatment cost between a computer-based cognitive rehabilitation teletherapy program and a face-to-face rehabilitation program.

The authors compared outcomes of 19 participants who received computer-based cognitive teletherapy rehabilitation with 20 participants who received face-to-face speech-language rehabilitation. The study compared outcomes from 2 "real-word" treatment programs provided by an outpatient rehabilitation center. A total of 39 participants with moderate to severe closed head traumatic brain injuries and a minimum of 1 year following injury were analyzed. Outcome measures included clinical indicators of independent living status, return to work or school, and independent driving. Cost measures included the total cost of the treatment and a measure of service costs per hour. Time since injury was a covariate, and an analysis of covariance revealed no differences between groups in independent living, driving status, return to work or school, or total treatment costs. The computer-based teletherapy cognitive rehabilitation program provided similar functional outcomes as face-to-face speech-language therapy at a similar total cost. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Investigating driver willingness to drive through flooded waterways

Approximately 40% of all drowning deaths involve a motor vehicle. Regardless of its significance as a cause of flood-related mortality, there is continued prevalence of driving through flooded waterways in Australia and worldwide. We aimed to understand the motivational determinates of driving through flooded waterways in low and high-risk scenarios by utilizing an augmented theory of planned behaviour (TPB) with behavioural willingness as the outcome variable as well as the influence of additional predictors; namely perceived risk and past behaviour. Participants (n = 174; M_age = 27.43, SD = 10.76) answered standard TPB-based questions in regards to attitudes, subjective norm, and perceived behavioural control (PBC), as well as additional variables of perceived risk (i.e., perceived susceptibility and perceived severity) and past behaviour. Support was found for the augmented TPB as attitude, subjective norm, and PBC predicted behavioural willingness. Support was also found for perceived severity in the high-risk but not the low-risk scenario. No support was found for perceived susceptibility. Past behaviour emerged as a significant predictor of willingness in the low and high-risk scenario. The findings provide support for an augmented TPB in understanding individuals’ willingness to drive through flooded waterways, suggesting that a multi-strategy approach may be critical in attempts to reduce the incidence of such risky driving behaviour.